Try the Workflow Configurator. A convenient tool to build experimental workflows and find products to match your needs. Log Out. Sample type. Starting material. Amplicon size. Animal pathogen detection. Assay type. Cells transfected. Cleanup application. DNA fragment size. DNA methylation analysis.
Dye included. Gene analyzed. PDF 77KB. PDF 2MB. Safety Data Sheets 1. Safety Data Sheets EN. Articles EN Managing mortality: how cells regulate telomerase activity EN. EN The role of inflammation in cancer EN. EN Chemokines: manipulators of metastasis EN.
EN Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain EN. EN What enhances the inflammatory response: new players and unexpected roles EN.
EN Lipid autophagy in obesity and atherosclerosis: chewing the fat EN. EN Induced pluripotent stem cells: real stem cells? EN Silencing cell-cell communication to combat drug toxicity EN.
Download Files 3. XLS KB. Scientific Posters 1. Explore the RNA Universe! Kit Handbooks 1. Performance Data 7. Instrument Technical Documents 2. For gene expression and genomic analysis. For pathway-focused gene expression analysis. White Papers 2. Gene Expression Analysis 1.
Sphingosine kinase 1 expressed by endothelial colony-forming cells has a critical role in their revascularization activity. FBXW7 mutations in melanoma and a new therapeutic paradigm. Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them?
The best way to set the threshold is to make sure that your PPC values are between 18 and How many housekeeping genes are included in each PCR Array? Each PCR Array has 5 housekeeping genes. You can use one or an average of the most stable ones to do data analysis. There are less than a dozen genes encoded by the mitochondrial genome all other mitochondrial proteins are encoded by nuclear genes , and they are all transcribed as one transcript just like any prokaryote , so distinguishing the expression of individual genes by real-time RT-PCR is not possible.
It simultaneously profiles the expression of 84 pathway-specific genes, and five housekeeping genes. What are the guidelines for choosing a housekeeping gene for normalizing qPCR results? How can I ensure that reaction volume is not lost due to evaporation during thermal cycling? Be sure to carefully and completely seal the qPCR assay plate with fresh, optical, thin-wall, 8-cap strips or adhesive optical film before the plate is placed into the real-time cycler.
In addition, refer to your instrument's user's manual to determine whether the real-time cycler manufacturer recommends use of a plate compression pad during the run.
Do you always run samples in triplicates? Whether or not you run a sample in triplicate is determined by experimental setup and what you are going to use the data for.
Is it good to pool multiple RNA replicates to detect expression changes that are consistently reproducible? Pooling RNA from different sources should only be done when there is not enough sample. We recommend running biological replicates. To minimize the risk of contaminating your experiment with extraneous DNA, the following steps should be taken: Remove a single aliquot of water from your PCR-grade stock, sufficient to complete the experiment.
This minimizes the number of times that the stock container is opened, thereby minimizing contamination risks. Do I need to run a standard curve before the actual PCR array experiment? What are the guidelines for choosing a housekeeping gene for normalizing qPCR results? How can I ensure that reaction volume is not lost due to evaporation during thermal cycling? What is the delta Rn value? Why are my qPCR Ct values too low. May I try the data analysis tool without using your PCR array kit?
How do you determine the efficiency using the PCR array? Can I manually set the threshold line? Do you always run samples in triplicates? How many housekeeping genes are included in each PCR Array? Is it best to go through all of them, looking for a range of best fit, and then just choose one value that fits all of them? FAQ ID - EN Chemokines: manipulators of metastasis.
EN Chronic viral infection: reviving the immune response. EN DNA damage repair — regulation through autophagy. EN Silencing cell-cell communication to combat drug toxicity. EN The role of inflammation in cancer.
EN What enhances the inflammatory response: new players and unexpected roles. EN Induced pluripotent stem cells: real stem cells? EN Lipid autophagy in obesity and atherosclerosis: chewing the fat. EN Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain. EN Managing mortality: how cells regulate telomerase activity. Instrument Technical Documents. For pathway-focused gene expression analysis.
For gene expression and genomic analysis.
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